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    <title>Bio3DEM - 新手学习</title>
    <link>http://www.bio3dem.com/forum.php?mod=forumdisplay&amp;fid=48</link>
    <description>Latest 20 threads of 新手学习</description>
    <copyright>Copyright(C) Bio3DEM</copyright>
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      <title>Bio3DEM</title>
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    <item>
      <title>请问Caltech，Prof. Grant J. Jensen开的视频课大家可以打开吗？</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=253</link>
      <description><![CDATA[https://www.coursera.org/learn/cryo-em/lecture/IM2qK/introduction-to-the-course

不知道为什么，视频不能播放，之能看到下面的文字。]]></description>
      <category>新手学习</category>
      <author>Mentalist</author>
      <pubDate>Tue, 31 Oct 2017 01:00:37 +0000</pubDate>
    </item>
    <item>
      <title>【和小白一起学电镜之N】电镜制样方法</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=246</link>
      <description><![CDATA[mark]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Sat, 14 Oct 2017 14:32:51 +0000</pubDate>
    </item>
    <item>
      <title>关于pixel size 引起的map的问题</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=245</link>
      <description><![CDATA[[*]怎么能把不同pixel不同分辨率的refine好的density map做个normalization呢（怎么比较两个相对独立的pixel size不同的map的区别）？可以接受用个低分辨率的threshold～
       答：Chimera 里面打开多个不同pixel size的相同结构 本来就是重合在一起的 跟pixel size ...]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Thu, 12 Oct 2017 13:37:49 +0000</pubDate>
    </item>
    <item>
      <title>pandora bracelets charms cheap  do not underestimate the small accessories</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=237</link>
      <description><![CDATA[This figure is slightly plump.the and you can also choose the style of the times to make you more attractive.
  Post documents to BlogDisclaimer: This article is written by the author who has been assigned to the Sohu the view only represents the aut ...]]></description>
      <category>新手学习</category>
      <author>qelYcn8jo</author>
      <pubDate>Tue, 15 Aug 2017 01:19:59 +0000</pubDate>
    </item>
    <item>
      <title>imitation cartier necklaces</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=236</link>
      <description><![CDATA[ear rings) (ring necklace brooch) four sets of ornaments: (ring necklace earrings wallpaper and decoration style Silver Necklace in the law, Therefore, new house decorations pictures new house decorations collocation skills 1,I G x E When the 2. the  ...]]></description>
      <category>新手学习</category>
      <author>qelYcn8jo</author>
      <pubDate>Tue, 15 Aug 2017 01:17:04 +0000</pubDate>
    </item>
    <item>
      <title>manual particle picking in relion1.3</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=233</link>
      <description><![CDATA[各位好，本人刚开始转入电镜领域，现在在用relion1.3和relion-2.0练习。在用relion－1.3做完manual picking后再做particl extract 时出现错误。意思是XXX_manual.star文件不存在。可是在文件夹Micrograph里是存在的，不知道是什么原因。请各位帮忙解答下，不胜感激。
 ...]]></description>
      <category>新手学习</category>
      <author>xmq234</author>
      <pubDate>Sat, 05 Aug 2017 02:27:06 +0000</pubDate>
    </item>
    <item>
      <title>the bible of single-particle cryo-EM</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=227</link>
      <description><![CDATA[《Three-Dimensional Electron Microscopy of Macromolecular Assemblies》
\&quot;Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction... The book gives an excellen ...]]></description>
      <category>新手学习</category>
      <author>admincryoem</author>
      <pubDate>Thu, 03 Aug 2017 03:09:22 +0000</pubDate>
    </item>
    <item>
      <title>phase plate 的 一个视频报告</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=218</link>
      <description><![CDATA[由Rado 在法国2017年的冷冻电镜会议上做的报告。

https://www.youtube.com/watch?v=TIv_RkzKj9E&amp;list=PLsWatK2_NAmxc4x4Fy4OiXcMVXNNDicje&amp;index=12]]></description>
      <category>新手学习</category>
      <author>admincryoem</author>
      <pubDate>Mon, 24 Jul 2017 03:19:06 +0000</pubDate>
    </item>
    <item>
      <title>对不起，论坛被黑，向大家道歉！</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=216</link>
      <description><![CDATA[对不起，论坛被黑，向大家道歉！]]></description>
      <category>新手学习</category>
      <author>Jack_Zhang</author>
      <pubDate>Sat, 22 Jul 2017 14:43:05 +0000</pubDate>
    </item>
    <item>
      <title>【和小白一起学电镜】之二：Basic anatomy of the EM</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=44</link>
      <description><![CDATA[电镜一般由以下几个系统构成：电子光学系统、真空系统、控制系统、相机以及图像收集系统构成。

这一部分我们以电子光学系统为主～

以TEM: transmission electron microscopy（TEM 透射电镜）为例，下图为从web上download的图片～ 左图为FEI公司的TECNAI 电镜，右图为 ...]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Mon, 26 Jun 2017 08:38:03 +0000</pubDate>
    </item>
    <item>
      <title>【和小白一起学电镜之一】Why electrons</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=39</link>
      <description><![CDATA[【和小白一起学电镜】系列一周一更新，欢迎大家一起学习，一起讨论～～学习内容主要以Grant J.Jensen的课为主～～

Part 1. Why electrons：

Different Raditions‘ advantages and disadvantages:

（图片来自于Grant J.Jensen视频）
Questions: 上课内容提及到Zone p ...]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Sat, 17 Jun 2017 05:45:37 +0000</pubDate>
    </item>
    <item>
      <title>Cryo-EM 初学者入门书籍和视频（慢慢整理中）</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=38</link>
      <description><![CDATA[欢迎大家提供补充：【本楼层会归纳整理后续新楼层的推荐】【类别会慢慢改进～】

书籍【仅用于学习交流，下载后请于24h内删除，禁止外传以及用于商业活动】：

冷冻电镜相关书籍：
《Three-Dimensional Electron Microscopy of Macromolecular Assemblies》by Joachim F ...]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Sat, 17 Jun 2017 05:32:57 +0000</pubDate>
    </item>
    <item>
      <title>冷冻制样问题集锦</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=34</link>
      <description><![CDATA[1.  图示中这样的grid改向什么方向优化啊，试了几种不同的glow时间，blot时间，样品load量，最后结果都差别不大。       样品为：膜蛋白，处于detergent里，用vitrobot冻的。



   出现原因：冰太薄，有detergent ， blot的时候溶液跑得太快了，一下子就blot干了   解 ...]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Tue, 13 Jun 2017 16:00:35 +0000</pubDate>
    </item>
    <item>
      <title>Cryo-EM 常见问题及解决方法【可以提问，请勿灌水，谢谢！】</title>
      <link>http://www.bio3dem.com/forum.php?mod=viewthread&amp;tid=23</link>
      <description><![CDATA[1. 如何把.mrc 文件转化为.mtz文件？    答：命令phenix.map_to_structure_factors 
2. 有没有程序可以refine model的local b factor？ 答：refmac
3. 一个是extract出来的raw particle，另外一个数目少的是经过多轮处理之后的好的particle，为什么即使是多轮处理之后好 ...]]></description>
      <category>新手学习</category>
      <author>Somnus8805</author>
      <pubDate>Mon, 12 Jun 2017 06:08:40 +0000</pubDate>
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